A simple strategy for the separation and purification of water-soluble polysaccharides from the fresh Spirulina platensis

نویسندگان

  • Feng Wang
  • Yong Ma
  • Yanhua Liu
  • Zhenggang Cui
  • Xiaoyan Ying
  • Fuming Zhang
  • Robert J. Linhardt
چکیده

A novel and industrially applicable strategy for separating and purifying the water-soluble polysaccharides from the fresh Spirulina platensis is introduced in this study. After hot water extraction, using chitosan flocculation treatment, 86.0% of the particles and 85.9% of the pigments in the crude extract were removed. The purity of polysaccharides could be increased to 94.6% and 96.1% by applying the macroporous resin ADS-7 to remove the impurities in the mode of static adsorption and in a dynamic mode of operation, respectively. The polysaccharides obtained were composed of three major components having molecular weights of 1400, 420 and 2 kDa. ARTICLE HISTORY Received 18 July 2016 Accepted 30 September 2016

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تاریخ انتشار 2017